Complement C4 (serum)

Complement C4 increases modestly during pregnancy because of estrogen-stimulated hepatic synthesis and physiologic complement activation.

Units Nonpregnant Adult 1st Trimester 2nd Trimester 3rd Trimester
mg/dL 16 – 47 9 – 45 10 – 42 17 – 37
g/L 0.16 – 0.47 0.09 – 0.45 0.10 – 0.42 0.17 – 0.37
Pregnancy physiology
  • Mild physiologic increase due to estrogen-mediated hepatic stimulation.
  • Part of global complement activation that supports placental and immune function.
  • Values peak mid-pregnancy and decline slightly in late gestation.
Causes of decreased C4
  • Systemic lupus erythematosus (SLE)
  • Lupus nephritis (especially proliferative disease)
  • Hereditary angioedema (C1 inhibitor deficiency — hallmark: low C4, normal C3)
  • Hereditary complement deficiencies
  • Immune complex glomerulonephritis
  • Severe liver disease
  • Sepsis or massive complement activation

Falling C4 in pregnancy may indicate lupus flare, complement consumption, or hereditary angioedema.

Causes of increased C4
  • Normal physiologic rise in pregnancy
  • Acute or chronic inflammation
  • Obesity and metabolic syndrome
  • Hyperestrogenic states (pregnancy, OCPs)
  • Corticosteroid therapy
  • Lymphoproliferative disorders (less common)

Elevated C4 usually reflects inflammation or estrogen exposure rather than complement activation.

References

  1. Lockitch G. Handbook of Diagnostic Biochemistry and Hematology in Normal Pregnancy. CRC Press; 1993.
  2. Abbassi-Ghanavati M, Greer LG, Cunningham FG. Pregnancy and laboratory studies: a reference table for clinicians. Obstet Gynecol. 2009;114:1326–31.
  3. Wallach J. Interpretation of Diagnostic Tests. 8th ed. Lippincott Williams & Wilkins; 2007.
  4. Gronowski AM (ed). Handbook of Clinical Laboratory Testing During Pregnancy. Humana Press; 2004.